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Application of the BIOMED ‐2 standardized primer system for the diagnosis of primary gastric B‐cell non‐Hodgkin's lymphoma
Author(s) -
Jia CongWei,
Sun Jian,
Gu YuMei,
Zhang TingTing,
Liu MingYang,
Chen Jie,
Lu ZhaoHui
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12250
Subject(s) - lymphoma , medicine , b cell , primer (cosmetics) , chronic gastritis , pathological , differential diagnosis , pathology , b cell lymphoma , gastric biopsy , gastritis , biopsy , gastroenterology , antibody , stomach , immunology , chemistry , organic chemistry
Primary gastric B‐cell non‐Hodgkin's lymphoma (B‐ NHL ) has a similar morphocytological presentation to severe chronic gastritis, which complicates the pathological diagnosis of this disease. To investigate the practicality and utility of the BIOMED ‐2 standardized primer system for the diagnosis of primary gastric B‐ NHL from endoscopic biopsy specimens, we selected 65 cases of archived paraffin‐embedded primary gastric B‐ NHL specimens as well as 27 cases of severe chronic gastritis samples to serve as a negative control group. The positivity rates of immunoglobulin heavy chain (IgH) gene rearrangements detected by the BIOMED ‐2 standardized primer system for the two groups were 86.4% and 12.0%, respectively, which are significantly different (p < 0.05). Importantly, the combined detection of the five groups of the IgH primer system increased the detection rate of B‐ NHL . These findings indicate that the BIOMED ‐2 standardized primer system is suitable for formalin‐fixed and paraffin‐embedded (FFPE) specimens and is valuable as a secondary diagnostic tool for primary gastric B‐ NHL as well as the differential diagnosis of severe chronic gastritis.