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Diversity of bla genes and low incidence of CTX ‐M in plasmid‐mediated AmpC‐producing Escherichia coli clinical isolates
Author(s) -
GalánSánchez Fátima,
AznarMarín Pilar,
MarínCasanova Pilar,
RodríguezIglesias Manuel
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12214
Subject(s) - microbiology and biotechnology , biology , multiplex polymerase chain reaction , escherichia coli , plasmid , strain (injury) , broth microdilution , antimicrobial , gene , polymerase chain reaction , genetics , minimum inhibitory concentration , anatomy
The aim of this study was to characterize plasmid‐mediated AmpC ( pA mpC)‐producing E scherichia coli clinical isolates. A total of 101 strains with AmpC‐susceptibility pattern were prospectively included. All isolates were tested by multiplex PCR to detect different bla genes. Phylogenetic groups were determined by a multiplex PCR assay. Antimicrobial susceptibility was tested by a microdilution commercial method. Presence of bla pAmpC was detected in 79 (78.2%) of the strains; in these pA mpC‐producing isolates, bla TEM was detected in 41 (51.9%) strains, bla SHV in 5 (6.3%) strains, bla OXA in 3 (3.8%) strains, and bla CTX‐M in 3 (3.8%) strains. bla VIM and bla KPC were detected in one strain. Sixteen strains belonged to phylogroup A, 27 to B1, 20 to B2, and 16 to D. As conclusion, the majority of the strains of E. coli with AmpC‐susceptibility pattern are pAmpC positive, although the association of extended‐spectrum beta‐lactamases ( ESBL ) and pAmpC is unusual.