Premium
Procedure for microbial identification based on Matrix‐Assisted Laser Desorption/Ionization‐Time of Flight Mass Spectrometry from screening‐positive urine samples
Author(s) -
March Rosselló Gabriel A.,
Gutiérrez Rodríguez María P.,
Lejarazu Leonardo Raúl Ortiz,
Orduña Domingo Antonio,
Bratos Pérez Miguel A.
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12208
Subject(s) - mass spectrometry , chromatography , urine , surface enhanced laser desorption/ionization , matrix assisted laser desorption/ionization , chemistry , matrix (chemical analysis) , desorption , time of flight mass spectrometry , laser , ionization , identification (biology) , analytical chemistry (journal) , sample preparation in mass spectrometry , biology , biochemistry , physics , electrospray ionization , ion , optics , organic chemistry , botany , adsorption
Matrix‐Assisted Laser Desorption/Ionization‐Time of Flight Mass Spectrometry is a widely used proteomic technique in clinical microbiology laboratories, and enables microbial identification directly from clinical samples. This study seeks to establish a protocol for bacterial identification from monomicrobial urine samples that have tested positive in the screening with Sysmex UF‐1000i (Sysmex Corporation, Kobe, Japan). Sysmex UF‐1000i counts ≥1 × 10 7 bacteria/mL indicate a sufficient bacterial concentration to allow direct identification from urine, with 87.5% sensitivity. Microbial identification from urine with Sysmex UF‐1000i counts between 1 × 10 5 and 1 × 10 7 bacteria/ml requires preincubation to obtain the adequate amount of bacteria needed for analysis, and 91.7% sensitivity thus being achieved.