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Predominance of IncL/M and IncF plasmid types among CTX ‐M‐ ESBL ‐producing E scherichia coli and K lebsiella pneumoniae in B ulgarian hospitals
Author(s) -
Markovska Rumyana,
Schneider Ines,
Ivanova Dobrinka,
Mitov Ivan,
Bauernfeind Adolf
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12204
Subject(s) - plasmid , replicon , klebsiella pneumoniae , biology , microbiology and biotechnology , escherichia coli , typing , lineage (genetic) , genetics , gene
Our objective was to investigate the plasmid replicon‐types involved in spread of ESBL s among B ulgarian K lebsiella pneumoniae and E scherichia coli . Sixty‐three isolates, with transferable beta‐lactam resistance determinants, collected between 2007 and 2009 in six medical institutions, were analysed with respect to their antimicrobial susceptibility, ESBL ‐, RAPD ‐, and plasmid replicon‐type. Phylogenetic typing and screening for the O25b‐ ST 131 lineage were carried out for E . coli . The predominant ESBL s were CTX ‐M‐15 (81%) among E . coli and CTX ‐M‐3 (58%) among K . pneumoniae . Other sporadically found ESBL s were SHV ‐12 and TEM ‐139, and for the first time in B ulgaria, CTX ‐M‐1 and CTX ‐M‐14. Replicon typing revealed that plasmids carrying bla CTX‐M‐3 exclusively belonged to IncL/M‐type, while bla CTX‐M‐15 was predominantly (94%) associated with IncF‐type plasmids. Among E . coli, 59% of the isolates were clonally related. Isolates of that cluster produced CTX ‐M‐15, belonged to the O25b‐ ST 131 lineage, predominantly harboured plasmids with the FIA replicon, and were found in five centres. Among CTX ‐M‐3‐producing K . pneumoniae, two prevailing RAPD ‐types were found, one remained restricted to one centre and the second was found in three centres. The incompatibility groups IncN and IncA/C linked with bla SHV‐12 respectively bla TEM‐139 were found only once. To the best of our knowledge, this is the first detailed investigation of plasmids carrying ESBL genes among B ulgarian isolates demonstrating wide distribution of conjugative IncF plasmids among CTX ‐M‐15‐producing E . coli and IncL/M plasmids among CTX ‐M‐3 positive K . pneumoniae isolates.