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High‐risk human papillomavirus load and biomarkers in cervical intraepithelial neoplasia and cancer
Author(s) -
Chang Mee Soo,
Oh Sohee,
Jung EunJung,
Park Jeong Hwan,
Jeon HyeWon,
Lee Taek Sang,
Kim Jung Ho,
Choi Euno,
Byeon SunJu,
Park InAe
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12163
Subject(s) - cervical intraepithelial neoplasia , medicine , cervical cancer , biopsy , oncology , dysplasia , odds ratio , cancer , hpv infection , gastroenterology , pathology , gynecology
The purpose of this study was to examine the implication of high‐risk human papillomavirus ( HPV ) load in cervical intraepithelial neoplasia ( CIN ) and cancer, and to detect biomarkers in cervical disease. We conducted high‐risk HPV DNA load and cervical cytology tests in 343 women, cervical tissue biopsy in 143 women, and immunohistochemistry for p16 INK4A , cyclin D1, p53, cyclooxygenase‐2, Ki‐67, GLUT1, hP ygopus2, and beta‐catenin. As a result, HPV load [relative light units (RLU) value] was correlated with the histological severity of cervical disease (p < 0.05). In the ‘atypical squamous cells of undetermined significance’ cytology group, 2.385 of HPV load seemed to be the cut‐off value at which ‘benign’ or CIN I can be differentiated from ‘CIN II or more severe’ (AUC = 0.712), but not statistically significant. The relative risk (odds ratio) of p16 INK4A and GLUT1 overexpression increased gradually according to the histological severity of cervical disease. The p16 INK4A showed statistically significant odds ratios in CIN II, CIN III, and cancer; GLUT1, in CIN II and CIN III; hPygopus2, in CIN III; and beta‐catenin, in CIN III and cancer. Conclusively, HPV load, p16 INK4A , and GLUT1 can be instrumental in predicting the severity of HPV‐related cervical disease. The beta‐catenin/hPygopus2 signaling may be involved in proceeding to CIN III.