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Molecular characterization and antibiotic resistance of clinical isolates of methicillin‐resistant Staphylococcus aureus obtained from Southeast of Iran (Kerman)
Author(s) -
Sadeghi Javid,
Mansouri Shahla
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12158
Subject(s) - sccmec , microbiology and biotechnology , clindamycin , staphylococcus aureus , methicillin resistant staphylococcus aureus , typing , biology , gentamicin , linezolid , vancomycin , antibiotic resistance , ciprofloxacin , erythromycin , multiplex polymerase chain reaction , antibiotics , virology , polymerase chain reaction , gene , bacteria , genetics
Staphylococcus aureus infections, particularly infections caused by methicillin‐resistant S. aureus ( MRSA ) strains, are emerging as a major public health problem. The aim of this study was to determine the prevalence of MRSA , antibiotic resistance profile and staphylococcal cassette chromosome mec ( SCC mec ) type of MRSA isolates obtained from clinical samples. Totally, 162 S. aureus isolates were obtained from clinical samples at three university hospitals in Kerman, Iran from March 2011 to February 2012. All isolates were identified as S. aureus by phenotypic methods and confirmed by PCR amplification of the nuc gene . MRSA isolates were screened by phenotypic tests and confirmed by presence of mec A gene. The minimum inhibitory concentrations ( MIC s) of the MRSA isolates against antibacterial agents were determined by E‐test. All isolates were analyzed by PCR for the presence of mec A and pvl genes. SCC mec typing of MRSA isolates was performed by multiplex PCR assay. Strain typing was carried out with REP ‐ PCR . Using mec A gene PCR and phenotypic methods, 56.8% of the isolates were identified as MRSA . All MRSA isolates were susceptible to vancomycin and linezolid. The sensitivity of MRSA isolates to trimethoprim‐sulfamethoxazole, clindamycin, ciprofloxacin, gentamicin, and erythromycin was 70.66, 66.53, 42.4, 38.05, and 29.35%, respectively. The most frequent SCC mec types were type III (48.31%) followed by type V (19.1%), type I (16.85%), and type IV (3.37%). The pvl gene was detected in 3.08% of isolates (two MRSA and three MSSA isolates). REP ‐ PCR typing divided the 92 MRSA isolates into 10 distinct clusters. Our results indicate that vancomycin and linezolid are the most effective antibacterial agents against MRSA isolates and SCC mec type III is predominant in MRSA strains in this area.