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Molecular microbiological evaluation of subgingival biofilm sampling by paper point and curette
Author(s) -
Belibasakis Georgios N.,
Schmidlin Patrick R.,
Sahrmann Philipp
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12151
Subject(s) - curette , biofilm , dentistry , sampling (signal processing) , microbiology and biotechnology , medicine , biology , bacteria , computer science , pathology , computer vision , genetics , filter (signal processing)
The present clinical study aimed to investigate if there are differences in microbiological outcomes dependent on the subgingival biofilm collection method. Subgingival biofilm samples were collected from the four deepest pockets (>5 mm) of 17 patients with aggressive periodontitis (AgP) and 33 patients with chronic periodontitis ( CP ), first by paper point and thereafter by curette. Samples obtained with the same method were pooled together from each patient and forwarded for molecular microbiological analysis by a commercially available assay ( IAI Pado Test 4.5) that estimates total bacterial load and levels of P orphyromonas gingivalis , T annerella forsythia , T reponema denticola and A ggregatibacter actinomycetemcomitans . Data analysis included frequency of detection, quantification and correlation of detection levels between the two sampling methods. P . gingivalis , T . forsythia and T . denticola were detected in >90% of the samples, and their detection levels exhibited a strong correlation between sampling methods. The detection consistency of A. actinomycetemcomitans was 56% between the two sampling methods. A. actinomycetemcomitans was more readily detected by paper point compared with curette collection with a stronger correlation between the two methods in AgP. Subgingival biofilm sampling by curette or paper point does not yield differences in the detection of the three ‘red complex’ species. However, A. actinomycetemcomitans was more consistently detected by means of paper point collection, which can be crucial in the decision to administer antibiotics as an adjunctive periodontal treatment.