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Enteric gram‐negative bacilli suppress Candida biofilms on Foley urinary catheters
Author(s) -
Samaranayake Y. H.,
Bandara H. M. H. N.,
Cheung B. P. K.,
Yau J. Y. Y.,
Yeung S. K. W.,
Samaranayake L. P.
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12098
Subject(s) - microbiology and biotechnology , candida albicans , pseudomonas aeruginosa , biofilm , escherichia coli , bacilli , biology , bacteria , chemistry , biochemistry , genetics , gene
Mixed Candida ‐bacterial biofilms in urinary catheters are common in hospitalized patients. (i) The aims of this study were to evaluate, quantitatively and qualitatively, the in vitro development of mono‐ and dual‐species biofilms ( MSB s and DSB s) of Candida albicans and two enteric gram‐negative bacilli ( EGNB ; Pseudomonas aeruginosa or Escherichia coli) on Foley catheter ( FC ) discs, (ii) to determine the biofilm growth in tryptic soy broth or glucose supplemented artificial urine ( AU ) and (iii) to assess the inhibitory effects of EGNB and their lipopolysaccharides ( LPS ) on Candida biofilm growth. The growth of MSB s and DSB s on FC discs was monitored by cell counts and SEM . The metabolic activity of LPS ‐treated Candida biofilms was determined by the XTT reduction assay. Candida albicans and EGNB demonstrated significant inter‐ and intra‐species differences in biofilm growth on FC discs (p < 0.01). Pseudomonas aeruginosa suppressed Candida albicans significantly (p < 0.001) in DSB s. Compared with MSB s, DSB of EGNB in glucose supplemented AU demonstrated robust growth. Escherichia coli and its LPS , significantly suppressed Candida biofilm growth, compared with Pseudomonas aeruginosa and its LPS (p < 0.001). Candida albicans and EGNB colonization in FC is significantly increased in AU with glucose, and variably modified by Escherichia coli , Pseudomonas aeruginosa and their corresponding LPS .