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A rapid, high‐throughput screening method for carriage of methicillin‐resistant S taphylococcus aureus
Author(s) -
Tunsjø Hege S.,
FollinArbelet Benoit,
Clausen Nina M.,
Ness Yngve,
Leegaard Truls M.,
Bemanian Vahid
Publication year - 2013
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12049
Subject(s) - carriage , staphylococcus aureus , methicillin resistant staphylococcus aureus , microbiology and biotechnology , turnaround time , medicine , biology , computer science , bacteria , pathology , genetics , operating system
Rapid screening of methicillin‐resistant Staphylococcus aureus ( MRSA ) colonization prior to hospital admittance is important to reduce nosocomial infections and health care costs. Molecular detection of mecA and S . aureus specific target genes has become widely established for this purpose. However, there are still limitations in potential for high‐throughput screening in the methods described. We have compared the time aspects and workload of four different DNA preparation platforms, resulting in an automated and simple MRSA screening method which combines two liquid handling systems and a simple lysis buffer. We have further transferred our in‐house dual real‐time PCR to a fast‐ PCR protocol, reducing the time and labour spent on these samples to a minimum.