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Mitochondrial oxygen affinity increases after sprint interval training and is related to the improvement in peak oxygen uptake
Author(s) -
Larsen Filip J.,
Schiffer Tomas A.,
Zinner Christoph,
Willis Sarah J.,
MoralesAlamo David,
Calbet Jose A.L.,
Boushel Robert,
Holmberg HansChrister
Publication year - 2020
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/apha.13463
Subject(s) - sprint , cardiorespiratory fitness , interval training , vo2 max , oxygen , mitochondrion , p50 , respiration , medicine , zoology , chemistry , cardiology , physical therapy , biology , heart rate , anatomy , biochemistry , blood pressure , organic chemistry , transcription factor , gene
Aims The body responds to exercise training by profound adaptations throughout the cardiorespiratory and muscular systems, which may result in improvements in maximal oxygen consumption (VO 2 peak) and mitochondrial capacity. By convenience, mitochondrial respiration is often measured at supra‐physiological oxygen levels, an approach that ignores any potential regulatory role of mitochondrial affinity for oxygen (p50 mito ) at physiological oxygen levels. Methods In this study, we examined the p50 mito of mitochondria isolated from the Vastus lateralis and Triceps brachii in 12 healthy volunteers before and after a training intervention with seven sessions of sprint interval training using both leg cycling and arm cranking. The changes in p50 mito were compared to changes in whole‐body VO 2 peak. Results We here show that p50 mito is similar in isolated mitochondria from the Vastus (40 ± 3.8 Pa) compared to Triceps (39 ± 3.3) but decreases (mitochondrial oxygen affinity increases) after seven sessions of sprint interval training (to 26 ± 2.2 Pa in Vastus and 22 ± 2.7 Pa in Triceps , both P < .01). The change in VO 2 peak modelled from changes in p50 mito was correlated to actual measured changes in VO 2 peak ( R 2 = .41, P = .002). Conclusion Together with mitochondrial respiratory capacity, p50 mito is a critical factor when measuring mitochondrial function, it can decrease with sprint interval training and should be considered in the integrative analysis of the oxygen cascade from lung to mitochondria.