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Disturbed cardiac mitochondrial and cytosolic calcium handling in a metabolic risk‐related rat model of heart failure with preserved ejection fraction
Author(s) -
MirandaSilva Daniela,
Wüst Rob C. I.,
Conceição Glória,
GonçalvesRodrigues Patrícia,
Gonçalves Nádia,
Gonçalves Alexandre,
Kuster Diederik W. D.,
LeiteMoreira Adelino F.,
Velden Jolanda,
Sousa Beleza Jorge M.,
Magalhães José,
Stienen Ger J. M.,
FalcãoPires Inês
Publication year - 2020
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/apha.13378
Subject(s) - heart failure with preserved ejection fraction , mitochondrion , medicine , cytosol , calcium , endocrinology , heart failure , mitochondrial ros , mitochondrial matrix , ejection fraction , biology , chemistry , biochemistry , enzyme
Abstract Aim Calcium ions play a pivotal role in matching energy supply and demand in cardiac muscle. Mitochondrial calcium concentration is lower in animal models of heart failure with reduced ejection fraction (HFrEF), but limited information is available about mitochondrial calcium handling in heart failure with preserved ejection fraction (HFpEF). Methods We assessed mitochondrial Ca 2+ handling in intact cardiomyocytes from Zucker/fatty Spontaneously hypertensive F1 hybrid (ZSF1)‐lean (control) and ZSF1‐obese rats, a metabolic risk‐related model of HFpEF. A mitochondrially targeted Ca 2+ indicator (MitoCam) was expressed in cultured adult rat cardiomyocytes. Cytosolic and mitochondrial Ca 2+ transients were measured at different stimulation frequencies. Mitochondrial respiration and swelling, and expression of key proteins were determined ex vivo. Results At rest, mitochondrial Ca 2+ concentration in ZSF1‐obese was larger than in ZSF1‐lean. The diastolic and systolic mitochondrial Ca 2+ concentrations increased with stimulation frequency, but the steady‐state levels were larger in ZSF1‐obese. The half‐widths of the contractile responses, the resting cytosolic Ca 2+ concentration and the decay half‐times of the cytosolic Ca 2+ transients were higher in ZSF1‐obese, likely because of a lower SERCA2a/phospholamban ratio. Mitochondrial respiration was lower, particularly with nicotinamide adenine dinucleotide (NADH) (complex I) substrates, and mitochondrial swelling was larger in ZSF1‐obese. Conclusion The free mitochondrial calcium concentration is higher in HFpEF owing to alterations in mitochondrial and cytosolic Ca 2+ handling. This coupling between cytosolic and mitochondrial Ca 2+ levels may compensate for myocardial ATP supply in vivo under conditions of mild mitochondrial dysfunction. However, if mitochondrial Ca 2+ concentration is sustainedly increased, it might trigger mitochondrial permeability transition pore opening.