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Atrial natriuretic peptide reduces inflammation and enhances apoptosis in rat acute pancreatitis
Author(s) -
Najenson A. C.,
Courreges A. P.,
Perazzo J. C.,
Rubio M. F.,
Vatta M. S.,
Bianciotti L. G.
Publication year - 2018
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/apha.12992
Subject(s) - atrial natriuretic peptide , medicine , pancreatitis , endocrinology , secretin , acute pancreatitis , inflammation , acinar cell , apoptosis , natriuretic peptide , pancreas , biology , heart failure , biochemistry
Aim We previously reported that atrial natriuretic peptide ( ANP ) reduces serum amylase and intrapancreatic trypsinogen activation in the onset of acute pancreatitis whereas secretin increases them. In the present work, we sought to establish the effect of ANP and secretin on the inflammatory response and cell death in experimental acute pancreatitis. Methods The expression and activity of key inflammatory mediators and apoptosis were evaluated in the presence or absence of the atrial peptide, secretin or both in cerulein‐induced acute pancreatitis in rats. Also, ultrastructural changes in pancreatic acinar cells were assessed by transmission electron microscopy. Results ANP significantly reduced NF ‐κB activation and TNF ‐α intrapancreatic levels. Furthermore, it decreased inducible nitric oxide synthase and cyclooxygenase 2 expression and activity while it diminished myeloperoxidase activity. ANP also stimulated apoptosis as shown by caspase‐3 expression and activation as well as TUNEL assay. These findings correlated well with the ultrastructural changes observed in the exocrine pancreas. Although secretin reduced various inflammatory markers, it also diminished caspase‐3 activation and the overall response was the aggravation of the disease as reflected by the ultrastructural alterations of pancreatic acinar cells. In the presence of ANP , various effects evoked by secretin were antagonized. Conclusion Present findings show that ANP significantly attenuated the severity of acute pancreatitis in the rat by inducing apoptosis and reducing the inflammatory response and further suggest that ANP may have eventual therapeutic implications in the disease and/or in medical interventions at risk of its developing like endoscopic retrograde cholangiopancreatography.