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Electrical pulse stimulation of cultured skeletal muscle cells as a model for in vitro exercise – possibilities and limitations
Author(s) -
Nikolić N.,
Görgens S. W.,
Thoresen G. H.,
Aas V.,
Eckel J.,
Eckardt K.
Publication year - 2017
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/apha.12830
Subject(s) - in vivo , stimulation , in vitro , skeletal muscle , muscle contraction , biology , contraction (grammar) , neuroscience , microbiology and biotechnology , medicine , anatomy , endocrinology , biochemistry
The beneficial health‐related effects of exercise are well recognized, and numerous studies have investigated underlying mechanism using various in vivo and in vitro models. Although electrical pulse stimulation ( EPS ) for the induction of muscle contraction has been used for quite some time, its application on cultured skeletal muscle cells of animal or human origin as a model of in vitro exercise is a more recent development. In this review, we compare in vivo exercise and in vitro EPS with regard to effects on signalling, expression level and metabolism. We provide a comprehensive overview of different EPS protocols and their applications, discuss technical aspects of this model including critical controls and the importance of a proper maintenance procedure and finally discuss the limitations of the EPS model.