Electrical pulse stimulation of cultured skeletal muscle cells as a model for  in vitro  exercise – possibilities and limitations | Zendy

Nikolić N. | Zendy; Görgens S. W. | Zendy; Thoresen G. H. | Zendy; Aas V. | Zendy; Eckel J. | Zendy; Eckardt K. | Zendy

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Electrical pulse stimulation of cultured skeletal muscle cells as a model for in vitro exercise – possibilities and limitations

Author(s) -

Nikolić N.,

Görgens S. W.,

Thoresen G. H.,

Aas V.,

Eckel J.,

Eckardt K.

Publication year - 2017

Publication title -

acta physiologica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.591

H-Index - 116

eISSN - 1748-1716

pISSN - 1748-1708

DOI - 10.1111/apha.12830

Subject(s) - in vivo , stimulation , in vitro , skeletal muscle , muscle contraction , biology , contraction (grammar) , neuroscience , microbiology and biotechnology , medicine , anatomy , endocrinology , biochemistry

The beneficial health‐related effects of exercise are well recognized, and numerous studies have investigated underlying mechanism using various in vivo and in vitro models. Although electrical pulse stimulation ( EPS ) for the induction of muscle contraction has been used for quite some time, its application on cultured skeletal muscle cells of animal or human origin as a model of in vitro exercise is a more recent development. In this review, we compare in vivo exercise and in vitro EPS with regard to effects on signalling, expression level and metabolism. We provide a comprehensive overview of different EPS protocols and their applications, discuss technical aspects of this model including critical controls and the importance of a proper maintenance procedure and finally discuss the limitations of the EPS model.

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