Premium
Dopamine enhances duodenal epithelial permeability via the dopamine D 5 receptor in rodent
Author(s) -
Feng X.Y.,
Zhang D.N.,
Wang Y.A.,
Fan R.F.,
Hong F.,
Zhang Y.,
Li Y.,
Zhu J.X.
Publication year - 2017
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/apha.12806
Subject(s) - endocrinology , medicine , duodenum , dopamine , tight junction , transgene , receptor , dextran , intestinal permeability , biology , chemistry , permeability (electromagnetism) , microbiology and biotechnology , biochemistry , membrane , gene
Aim The intestinal barrier is made up of epithelial cells and intercellular junctional complexes to regulate epithelial ion transport and permeability. Dopamine ( DA ) is able to promote duodenal epithelial ion transport through D1‐like receptors, which includes subtypes of D 1 (D 1 R) and D 5 (D 5 R), but whether D1‐like receptors influence the duodenal permeability is unclear. Methods FITC –dextran permeability, short‐circuit current ( I SC ), Western blot, immunohistochemistry and ELISA were used in human D 5 R transgenic mice and hyperendogenous enteric DA ( HE nD) rats in this study. Results Dopamine induced a downward deflection in I SC and an increase in FITC –dextran permeability of control rat duodenum, which were inhibited by the D1‐like receptor antagonist, SCH ‐23390. However, DA decreased duodenal transepithelial resistance ( TER ), an effect also reversed by SCH ‐23390. A strong immunofluorescence signal for D 5 R, but not D 1 R, was observed in the duodenum of control rat. In human D 5 R knock‐in transgenic mice, duodenal mucosa displayed an increased basal I SC with high FITC –dextran permeability and decreased TER with a lowered expression of tight junction proteins, suggesting attenuated duodenal barrier function in these transgenic mice. D 5 R knock‐down transgenic mice manifested a decreased basal I SC with lowered FITC –dextran permeability. Moreover, an increased FITC –dextran permeability combined with decreased TER and tight junction protein expression in duodenal mucosa were also observed in HE nD rats. Conclusion This study demonstrates, for the first time, that DA enhances duodenal permeability of control rat via D 5 R, which provides new experimental and theoretical evidence for the influence of DA on duodenal epithelial barrier function.