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Receptor‐interacting Protein 140 represses Sirtuin 3 to facilitate hypertrophy, mitochondrial dysfunction and energy metabolic dysfunction in cardiomyocytes
Author(s) -
You J.,
Yue Z.,
Chen S.,
Chen Y.,
Lu X.,
Zhang X.,
Shen P.,
Li J.,
Han Q.,
Li Z.,
Liu P.
Publication year - 2017
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/apha.12800
Subject(s) - sirtuin 1 , biology , sirtuin , mitochondrion , downregulation and upregulation , gene silencing , microbiology and biotechnology , medicine , endocrinology , gene , biochemistry , acetylation
Aim The transcriptional cofactor receptor‐interacting protein 140 ( RIP 140) is known as a deleterious regulator of cardiac mitochondrial function and energy metabolic homeostasis. This study revealed that RIP 140 repressed Sirtuin 3 ( SIRT 3), a mitochondrial deacetylase that plays an important role in regulating cardiac function. Methods RIP 140 was overexpressed by adenovirus infection or was knocked down by RNA interference in neonatal rat cardiomyocytes. Results RIP 140 overexpression repressed, while RIP 140 silencing elevated the expression and activity of SIRT 3. Ad‐ RIP 140 enhanced the expressions of the cardiac hypertrophic markers and increased cardiomyocyte surface area, whereas SIRT 3 overexpression prevented the effect of Ad‐ RIP 140. Additionally, SIRT 3 overexpression reversed Ad‐ RIP 140‐induced mitochondrial dysfunction and energy metabolic dysfunction, such as increase in oxidative stress, decrease in mitochondrial membrane potential and ATP production, as well as downregulation of mitochondrial DNA ‐encoded genes and genes related to mitochondrial genome replication and transcription, mitochondrial oxidative phosphorylation and fatty acid oxidation. In contrast, SIRT 3 silencing exacerbated RIP 140‐induced cardiomyocyte hypertrophy and mitochondrial dysfunction. Furthermore, the repression of SIRT 3 by RIP 140 was dependent on estrogen‐related receptor‐α ( ERR α). The involvement of peroxisome proliferator‐activated receptor‐γ coactivator‐1α ( PGC ‐1α) was ruled out of SIRT 3 suppression by RIP 140. RIP 140 and PGC ‐1α might act as functional antagonists on the regulation of SIRT 3. Conclusion This study indicates that suppression of SIRT 3 by RIP 140 facilitates the development of cardiomyocyte hypertrophy, mitochondrial dysfunction and energy metabolic dysfunction. Strategies targeting inhibition of RIP 140 and upregulation of SIRT 3 might improve cardiac energy metabolism and suggest therapeutic potential for heart diseases.