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Leptin into the ventrolateral medulla facilitates chemorespiratory response in leptin‐deficient (ob/ob) mice
Author(s) -
Bassi M.,
Furuya W. I.,
Menani J. V.,
Colombari D. S. A.,
do Carmo J. M.,
da Silva A. A.,
Hall J. E.,
Moreira T. S.,
Wenker I. C.,
Mulkey D. K.,
Colombari E.
Publication year - 2014
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/apha.12257
Subject(s) - leptin , medicine , endocrinology , ventilation (architecture) , respiratory system , microinjection , microinjections , control of respiration , hormone , tidal volume , obesity , mechanical engineering , engineering
Aim Leptin, an adipocyte‐derived hormone, is suggested to participate in the central control of breathing. We hypothesized that leptin may facilitate ventilatory responses to chemoreflex activation by acting on respiratory nuclei of the ventrolateral medulla. The baseline ventilation and the ventilatory responses to CO 2 were evaluated before and after daily injections of leptin into the retrotrapezoid nucleus/parafacial respiratory group ( RTN /p FRG ) for 3 days in obese leptin‐deficient (ob/ob) mice. Methods Male ob/ob mice (40–45 g, n = 7 per group) received daily microinjections of vehicle or leptin (1 μ g per 100 nL) for 3 days into the RTN /p FRG . Respiratory responses to CO 2 were measured by whole‐body plethysmography. Results Unilateral microinjection of leptin into the RTN /p FRG in ob/ob mice increased baseline ventilation ( V E ) from 1447 ± 96 to 2405 ±174 mL min −1 kg −1 by increasing tidal volume ( V T ) from 6.4 ± 0.4 to 9.1 ± 0.8 mL kg −1 ( P < 0.05). Leptin also enhanced ventilatory responses to 7% CO 2 (Δ = 2172 ± 218 mL min −1 kg −1 , vs. control: Δ = 1255 ± 105 mL min −1 kg −1 ), which was also due to increased V T (Δ = 4.71 ± 0.51 mL kg −1 , vs. control: Δ = 2.27 ± 0.20 mL kg −1 ), without changes in respiratory frequency. Leptin treatment into the RTN /p FRG or into the surrounding areas decreased food intake (83 and 70%, respectively), without significantly changing body weight. Conclusion The present results suggest that leptin acting in the respiratory nuclei of the ventrolateral medulla improves baseline V E and V T and facilitates respiratory responses to hypercapnia in ob/ob mice.