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Modulation of murine gastric vagal afferent mechanosensitivity by neuropeptide W
Author(s) -
Li H.,
Kentish S. J.,
Kritas S.,
Young R. L.,
Isaacs N. J.,
O'Donnell T. A.,
Blackshaw L. A.,
Wittert G. A.,
Page A. J.
Publication year - 2013
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/apha.12154
Subject(s) - nodose ganglion , medicine , endocrinology , receptor , retrograde tracing , gastric mucosa , neuropeptide , biology , stomach , chemistry , vagus nerve , central nervous system , stimulation
Aim Neuropeptide W ( NPW ) is an endogenous ligand for the receptors GPR 7 and GPR 8 and is involved in central regulation of energy homeostasis. NPW in the periphery is found in gastric gastrin (G) cells. In the stomach, energy intake is influenced by vagal afferent signals, so we aimed to determine the effect of NPW on mechanosensitive gastric vagal afferents under different feeding conditions. Methods Female C57 BL /6 mice ( N > 10 per group) were fed a standard laboratory diet ( SLD ), high‐fat diet ( HFD ) or were food restricted. The relationship between NPW immunopositive cells and gastric vagal afferent endings was determined by anterograde tracing and NPW immunohistochemistry. An in vitro gastro‐oesophageal preparation was used to determine the functional effects of NPW on gastric vagal afferents. Expression of NPW in the gastric mucosa and GPR 7 in whole nodose ganglia was determined by quantitative RT ‐ PCR ( QRT ‐ PCR ). The expression of GPR 7 in gastric vagal afferent neurones was determined by retrograde tracing and QRT ‐ PCR . Results Neuropeptide W immunoreactive cells were found in close proximity to traced vagal afferents. NPW selectively inhibited responses of gastric vagal tension receptors to stretch in SLD but not HFD or fasted mice. In the nodose ganglia, GPR7 mRNA was specifically expressed in gastric vagal afferent neurones. In fasted mice gastric mucosal NPW and nodose GPR7, mRNA was reduced compared with SLD. A HFD had no effect on gastric NPW mRNA , but down‐regulated nodose GPR7 expression. Conclusion Neuropeptide W modulates gastric vagal afferent activity, but the effect is dynamic and related to feeding status.