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The expression of heat shock protein in human skeletal muscle: effects of muscle fibre phenotype and training background
Author(s) -
Folkesson M.,
Mackey A. L.,
Langberg H.,
Oskarsson E.,
PiehlAulin K.,
Henriksson J.,
Kadi F.
Publication year - 2013
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/apha.12124
Subject(s) - hsp70 , skeletal muscle , heat shock protein , myosin , vastus lateralis muscle , hsp27 , hsp60 , endurance training , immunohistochemistry , medicine , endocrinology , muscle fibre , biology , microbiology and biotechnology , biochemistry , gene
Aim Exercise‐induced adaptations of skeletal muscle are related to training mode and can be muscle fibre type specific. This study aimed to investigate heat shock protein expression in type I and type II muscle fibres in resting skeletal muscle of subjects with different training backgrounds. Methods Three groups of subjects were included: healthy active not engaged in any training programme ( ACT , n = 12), resistance trained ( RES , n = 6) and endurance trained ( END , n = 8). Biopsies were obtained from vastus lateralis, and immunohistochemistry was performed using monoclonal antibodies against myosin heavy chain I and IIA , α B ‐crystallin, HSP 27, HSP 60 and HSP 70. Results In ACT and RES , but not in END , a fibre type–specific expression with higher staining intensity in type I than type II fibres was seen for α B ‐crystallin. The opposite ( II > I ) was found for HSP 27 in subjects from ACT (6 of 12 subjects) and RES (3 of 6), whereas all subjects from END displayed uniform staining. HSP 60 showed no fibre‐specific expression. HSP 70 displayed a fibre‐specific expression pattern (I > II) in ACT (4 of 12), but not in END or RES . Conclusion This study shows that the level of expression of the different HSPs in human skeletal muscle is influenced by muscle fibre phenotype. The fibre type–specific expression of HSP 70 is influenced by resistance and endurance training, whereas those of α B ‐crystallin and HSP 27 is influenced only by endurance training, suggesting the existence of a training‐modality‐specific action on the adaptive processes including heat shock proteins in human skeletal muscle.