Premium
Effects of Exogenous Insulin‐Like Growth Factor I on Insulin‐Like Growth Factor Binding Proteins in a Case of Growth Hormone Insensitivity (Laron‐Type)
Author(s) -
GOURMELEN M.,
PERIN L.,
BINOUX M.
Publication year - 1991
Publication title -
acta paediatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/apa.1991.80.s377.115
Subject(s) - endocrinology , medicine , insulin like growth factor binding protein , growth factor , basal (medicine) , insulin like growth factor , recombinant dna , somatomedin , insulin , blot , hormone , growth hormone deficiency , binding protein , growth hormone , receptor , biology , biochemistry , gene
The electrophoretic profiles of serum insulin‐like growth factor binding proteins (IGFBPs) from an 18‐year‐old patient with growth hormone (GH) insensitivity were analysed by Western ligand blotting before and after administration of recombinant human insulin‐like growth factor I (IGF‐I). Under basal conditions, the profile was the same as that observed in all cases of GH deficiency or insensitivity, that is, low IGFBP‐3 (seen as two bands of 41.5 and 38.5 kDa) and enhanced IGFBP‐2 and, to a lesser extent, IGFBP‐L Subcutaneous injection of IGF‐I, 40 μg/kg, provoked an increase in serum IGF‐I levels to close Jo the lower limits of the normal range and a small increase in IGFBP‐3, suggesting that IGF‐I has a direct effect on the synthesis of this GH/IGF‐I‐dependent IGFBP. Moderate increases were also observed in IGFBP‐2 and IGFBP‐4. Repeated doses of IGF‐I over 7 days had no further effect on these changes, which persisted for a few days after the last injection.