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MicroRNA profiling in Chinese children with Henoch‐Schonlein purpura and association between selected microRNAs and inflammatory biomarkers
Author(s) -
Li Jing,
Chen Meixue,
Wang Jinfeng,
Lu Lingling,
Li Xiang,
Le Yuan
Publication year - 2021
Publication title -
acta paediatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/apa.15789
Subject(s) - microrna , henoch schonlein purpura , medicine , immunology , flow cytometry , pathogenesis , biomarker , interleukin , microarray , cytokine , gene , biology , gene expression , genetics , disease , vasculitis
Aim This study aimed to profile the microRNA levels in Chinese Henoch‐Schonlein purpura (HSP) children and to explore their association with inflammatory factors and T helper 17 (Th17)/regulatory T (Treg). Methods Forty‐five HSP children and 27 healthy controls were enrolled in this study, and microRNA levels were profiled with a microRNA microarray. The levels of selected microRNAs were determined by quantitative real‐time PCR, and the levels of serum IgA, interleukin‐6, interleukin‐10 and interleukin‐17A were detected by enzyme‐linked immunosorbent assay. Additionally, Th17 and Treg cells were analysed by flow cytometry. Results There were 9 up‐regulated and 27 down‐regulated microRNAs in the PBMCs of Chinese HSP children. Among them, miR‐1‐3p, miR‐19b‐1‐5p and miR‐29b‐1‐5p were up‐regulated, while miR‐483‐5p and miR‐1246 were down‐regulated. Additionally, these selected microRNAs could differentiate HSP patients from healthy controls. Interestingly, miR‐29b‐1‐5p was correlated with IgA, miR‐19b‐1‐5p, miR‐483‐5p and miR‐1246 were correlated with interleukin‐6, while miR‐1‐3p and miR‐1246 were correlated with Th17/Treg. Conclusion This study reveals that the altered microRNAs could differentiate HSP from the healthy, and were associated with inflammatory factors or Th17/Treg. It is indicated that alteration in these microRNAs may contribute to the HSP pathogenesis and may become therapeutic targets or diagnostic biomarkers for HSP.