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Prevalence of extended‐spectrum beta‐lactamase‐producing E nterobacteriaceae in healthy S wedish preschool children
Author(s) -
Kaarme Johan,
Molin Ylva,
Olsen Björn,
Melhus Åsa
Publication year - 2013
Publication title -
acta paediatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/apa.12206
Subject(s) - feces , typing , medicine , carriage , beta lactamase , genotype , transmission (telecommunications) , epidemiology , antibiotics , escherichia coli , microbiology and biotechnology , molecular epidemiology , pediatrics , veterinary medicine , biology , gene , genetics , electrical engineering , pathology , engineering
Aim The objective was to determine the prevalence of extended‐spectrum beta‐lactamase ( ESBL )‐producing E nterobacteriaceae in faeces from healthy S wedish preschool children and to establish whether transmission took place between children in preschools. Methods Diapers from children attending preschools in U ppsala city were collected during S eptember to October 2010, and the faeces was cultured. Antibiotic profiles and carriage of CTX ‐M, TEM , SHV and A mp C type enzymes were determined. PCR ‐positive isolates were further characterized by sequencing and epidemiological typing. Statistics on antibiotic use and ESBL producers in paediatric patients at U ppsala U niversity H ospital were extracted for comparison. Results A total of 313 stool specimens were obtained, representing 24.5% of all preschool children in U ppsala city. The carriage rate of ESBL ‐producing E nterobacteriaceae was 2.9% among these healthy children. The corresponding figure for patients in the same age group was 8.4%. E scherichia coli with CTX ‐M type enzymes predominated, and only one E . coli isolate carried genes‐encoding CMY . CTX ‐M‐producing E . coli isolates with identical genotypes were found in children with no familial relation at two different preschools. Conclusions Using diapers, the prevalence of ESBL ‐producing E nterobacteriaceae in children was quickly established, and, most likely, a transmission of ESBL ‐producing E . coli was for the first time documented between children at the same preschool.