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Expression of interleukin ( IL )‐10 family cytokines in aqueous humour of patients with specific endogenous uveitic entities: elevated levels of IL ‐19 in human leucocyte antigen‐B27‐associated uveitis
Author(s) -
Abu ElAsrar Ahmed M.,
Berghmans Nele,
AlObeidan Saleh A.,
Gikandi Priscilla W.,
Opdenakker Ghislain,
Van Damme Jo,
Struyf Sofie
Publication year - 2019
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.14039
Subject(s) - proinflammatory cytokine , uveitis , medicine , immunology , sarcoidosis , hla b27 , tumor necrosis factor alpha , interleukin , interleukin 10 , antigen , cytokine , inflammation , human leukocyte antigen
Purpose Evidence exists that the interleukin ( IL )‐10 family of cytokines is involved in autoimmune diseases. The aim of this study was to analyse the levels of the IL ‐10 family cytokines IL ‐10, IL ‐19, IL ‐20, IL ‐22, IL ‐26, IL ‐28A and IL ‐29 in aqueous humour ( AH ) samples from patients with specific uveitic entities. In addition, we correlated their levels with the levels of the proinflammatory cytokines tumour necrosis factor‐ α ( TNF ‐ α ) and IL ‐1 β . Methods Aqueous humour ( AH ) samples from patients with active uveitis associated with Behçet's disease ( BD ; n = 13), sarcoidosis ( n = 8), human leucocyte antigen ( HLA )‐B27‐related inflammation ( n = 12), Vogt–Koyanagi–Harada ( VKH ) disease ( n = 12) and control subjects ( n = 9) were assayed with the use of a multiplex assay. Results Of all the IL ‐10 family cytokines studied, only IL ‐19 levels were significantly higher in AH samples of patients ( n = 45) than in controls (p = 0.022). When comparing the four individual disease groups to controls, IL ‐19 levels were only significantly higher in HLA ‐B27‐associated uveitis (p < 0.001). IL ‐19 levels were significantly higher in patients with HLA ‐B27‐associated uveitis than in patients with BD , sarcoidosis and VKH disease (p < 0.001; p = 0.002; p < 0.001, respectively). Significant correlations were found between AH levels of IL ‐19 and AH levels of TNF ‐ α , ( r = 0.3; p = 0.03) and IL ‐1 β ( r = 0.56; p < 0.001). Conclusions Among the IL ‐10 family cytokines analysed, IL ‐19 demonstrated the highest expression in endogenous uveitis, particularly in HLA ‐B27‐associated uveitis. IL ‐19 thus might assist in the regulation of inflammation in HLA ‐B27‐associated uveitis.