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Recombinant human serum albumin for corneal preservation
Author(s) -
Parekh Mohit,
Elbadawy Hossein,
Salvalaio Gianni,
Amoureux MarieClaude,
Di Iorio Enzo,
Fortier Denis,
Ponzin Diego,
Ferrari Stefano,
Ruzza Alessandro
Publication year - 2018
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.13498
Subject(s) - cornea , trypan blue , human serum albumin , eye bank , histology , ophthalmology , albumin , andrology , chemistry , medicine , apoptosis , pathology , biochemistry
Purpose To study the performance of a completely synthetic organ culture (OC) preservation system containing recombinant human serum albumin ( rHSA ) for preservation of human donor corneas. Methods Twenty‐four paired donor corneas were randomly collected, and one cornea from each donor was preserved in synthetic (experimental) and serum‐based media (control). The tissues were assessed at day 0; after 6 days of preservation at room temperature ( RT ) in Cornea Trans ® and Cornea Prep II ® ; after 28 days at 31°C in Cornea Syn ® [with rHSA ] and Cornea Max ® [with foetal calf serum (FCS)] and; 4‐day post deswelling in Cornea Trans ® and Cornea Jet ® . Thickness was determined with optical coherence tomography ( OCT ) and transparency with a validated, custom device. Morphology, endothelial cell density ( ECD ) and mortality were observed after treating the tissues with Trypan blue and sucrose. Glucose uptake by the cells was analysed. Data were compared using non‐parametric paired Wilcoxon tests with p < 0.05 deemed significant. Histology using periodic acid‐schiff ( PAS ), expressions of p63, CK 12, α SMA and ZO ‐1 were analysed, and cell apoptosis postpreservation was studied. Results Corneas stored in synthetic media showed a higher and statistically significant value as compared to serum‐based media in terms of viable endothelial cell density ( VECD ), mortality, morphology and glucose uptake postpreservation. Histology showed presence of all the layers, all the markers were expressed, and no apoptosis was observed in either media. Conclusion The new synthetic preservation system containing rHSA (and other confidential constituents) showed better preservation effects than traditional media containing serum.