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Diurnal expression of proteins in the retina of the blind cone‐rod homeobox ( Crx −/− ) mouse and the 129/Sv mouse: a proteomic study
Author(s) -
Møller Morten,
Rath Martin Fredensborg,
Ludvigsen Maja,
Honoré Bent,
Vorum Henrik
Publication year - 2017
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.13429
Subject(s) - circadian rhythm , biology , retina , circadian clock , retinal , microbiology and biotechnology , suprachiasmatic nucleus , visual phototransduction , per2 , clock , biochemistry , endocrinology , neuroscience
Purpose The vertebrate retina contains a circadian clock participating in adaptations to day and night vision. This peripheral clock is independent of the master clock in the suprachiasmatic nucleus (SCN). The retinal clock is located in several cell types, including the photoreceptors. To investigate the role of the circadian clock of the photoreceptor cells in regulation of retinal protein rhythms, we analysed diurnal protein expression in the photoreceptor‐deficient cone‐rod homeobox knockout mouse ( Crx −/− ) and the 129/Sv mouse. Methods 2D gels were made from retinal homogenates of 129/Sv and Crx −/− mice killed at midday and midnight. Stained gels were analysed by use of PDQ uest 2D gel analysis software. After trypsin digestion of differential expressed spots, the proteins were identified by LC ‐ MS / MS using a nano‐liquid chromatograph connected to a Q‐ TOF Premier mass spectrometer. These data were used to search the SWISS ‐ PROT database. Results Both the retinae of the control and the Crx −/− mice exhibited diurnal proteins rhythms. As expected, proteins involved in phototransduction were not detected in the Crx −/− mouse; in this phenotype, however, proteins from spots showing diurnal rhythms were specifically identified as enzymes involved in glucose metabolism, Krebs cycle, and mitochondrial enzymes. Data are available via ProteomeXchange with identifier PXD 005556. Conclusion We show diurnal protein rhythms in the retina of a mouse lacking the rods and cones. The diurnal protein rhythms in this genotype, lacking the circadian clock of the photoreceptors, might be caused by a circadian clock in other retinal cell types or a direct light input to the retina.