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Effect of femtosecond laser‐assisted lens surgery on posterior capsule opacification in the human capsular bag in vitro
Author(s) -
Wertheimer Christian,
Kreutzer Thomas C.,
Dirisamer Martin,
EiblLindner Kirsten,
Kook Daniel,
Priglinger Siegfried,
Mayer Wolfgang J.
Publication year - 2017
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.13103
Subject(s) - posterior capsule opacification , femtosecond , lens capsule , lens (geology) , ophthalmology , capsule , laser , medicine , cataract surgery , surgery , phacoemulsification , optics , biology , intraocular lens , physics , visual acuity , botany
Abstract Purpose To compare posterior capsule opacification (PCO) by observing lens epithelial cell growth in the human capsular bag in vitro between conventional lens surgery using phacoemulsification (Phaco) technique and femtosecond laser‐assisted lens surgery (FLACS). Methods For the in vitro human capsular bag model, 18 cadaver eyes from nine human donors underwent three types of lens surgery. Three groups consisting of six capsular bags were established, that is FLACS, Phaco and extracapsular lens extraction (ECCE). The capsular bag was transferred into equal cell culture conditions after using one of the defined surgical approaches. Cellular growth of lens epithelial cells was observed and photo‐documented. The time until full cell‐coverage of the capsular bag was measured. Results The human capsular bag model can be successfully prepared using FLACS. There was no statistically significant difference in time until cell‐coverage of the human donor capsular bag in vitro in all three surgical settings (ECCE versus Phaco p = 0.6; ECCE versus FLACS p = 1.0; Phaco versus FLACS p = 1.0). Conclusions In our in vitro human capsular bag model, we could not observe a statistically significant difference in PCO formation using different surgical approaches of lens extraction. Therefore, PCO formation might not be attributed to the type of surgery. Furthermore, this study shows that FLACS can be used for the human capsular bag model preparation and validates the human capsular bag model for future research.