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Modification of aquaporin expression in response to fenretinide‐induced transdifferentiation of ARPE ‐19 cells into neuronal‐like cells
Author(s) -
Salik Dany,
Motulsky Elie,
Gregoire Françoise,
Delforge Valérie,
Bolaky Nargis,
Caspers Laure,
Perret Jason,
Willermain François,
Delporte Christine
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.12837
Subject(s) - transdifferentiation , fenretinide , microbiology and biotechnology , aquaporin , biology , vimentin , western blot , chemistry , cell culture , retinoic acid , stem cell , retinoid , immunology , biochemistry , immunohistochemistry , genetics , gene
Purpose The goal of this study was to investigate the modifications of aquaporin ( AQP ) expression in ARPE ‐19 cells in response to fenretinide‐induced transdifferentiation into neuronal‐like cells Methods ARPE ‐19 cells were treated daily for 7 days with 3 μ m fenretinide or dimethyl sulphoxide as control. mRNA and protein expression were evaluated by real‐time quantitative PCR , Western blot analysis and immunofluorescence. Results Control ARPE ‐19 cells expressed AQP 1, AQP 4, AQP 6 and AQP 11 at the mRNA level, but only AQP 4, AQP 6 and AQP 11 at the protein level. Fenretinide induced the transdifferentiation of ARPE ‐19 cells into neuronal‐like cells. Indeed, fenretinide induced morphological changes similar to neurons characterized by elongated cell body and the formation of neurite branching. Moreover, ARPE ‐19 cells transdifferentiated to neuron‐like cells were characterized by significant decrease in retinal pigmented epithelium markers, for example cytokeratin 8 and cellular retinaldehyde‐binding protein, as well as an increase in neuronal markers such as synaptophysin and calretinin. AQP 4 expression, at both mRNA and protein levels, and AQP 6 expression, only at protein level, were significantly decreased in ARPE ‐19 cells transdifferentiated into neuronal‐like cells. Conclusions The expression of AQP 4 and AQP 6 is downregulated during fenretinide‐induced transdifferentiation.