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Increased intravitreal adenosine 5′‐triphosphate, adenosine 5′‐diphosphate and adenosine 5′‐monophosphate levels in patients with proliferative diabetic retinopathy
Author(s) -
Loukovaara Sirpa,
Sahanne Sari,
Jalkanen Sirpa,
Yegutkin Gennady G.
Publication year - 2015
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.12507
Subject(s) - adenosine , diabetic retinopathy , adenosine diphosphate , adenosine triphosphate , adenosine monophosphate , purine , vitrectomy , medicine , inosine , purine metabolism , pars plana , ophthalmology , diabetes mellitus , adenine nucleotide , chemistry , endocrinology , nucleotide , biochemistry , enzyme , platelet , visual acuity , platelet aggregation , gene
Abstract Purpose Extracellular purines play important role in ocular physiology, diabetes, vascular remodelling and adaptation to inflammation. This study was aimed to evaluate intravitreal purine levels in patients with diabetic retinopathy ( DR ) and other non‐vascular vitreoretinal eye diseases. Methods Vitreous samples were collected at the start of the three‐port pars plana vitrectomy. Study group comprised 55 eyes operated due to sight‐threatening forms of DR , including eyes of 24 patients with proliferative DR . Of the 143 non‐diabetic controls, 112 had rhegmatogenous retinal detachment and 31 macular hole or pucker. Intravitreal purine concentrations were determined using a combination of bioluminescent [adenosine 5′‐triphosphate ( ATP ), adenosine 5′‐diphosphate ( ADP )] and fluorometric [adenosine 5′‐monophosphate ( AMP ), adenosine, inosine] enzyme‐coupled sensing assays. Results Compared with non‐diabetic controls, DR eyes contained significantly higher (p < 0.01) concentrations of ATP (4.2 ± 0.6 versus 34.5 ± 13.7 n m ; mean ± SEM ), ADP (19.5 ± 2.7 versus 43.7 ± 14.5 n m ) and AMP (1290 ± 115 versus 1876 ± 190 n m ). Intravitreal adenosine and inosine levels varied within submicromolar to low micromolar range, and their concentrations did not differ between the groups studied. Conclusions High concentrations of intravitreal nucleotides ATP , ADP and AMP may be related to the pathogenesis of sight‐threatening forms of DR .