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Specific spatial distribution of caspase‐3 in normal lenses
Author(s) -
Talebizadeh Nooshin,
Yu Zhaohua,
Kronschläger Martin,
Hallböök Finn,
Söderberg Per
Publication year - 2015
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.12501
Subject(s) - caspase 3 , sagittal plane , lens (geology) , labelling , caspase 9 , epithelium , immunohistochemistry , caspase , apoptosis , distribution (mathematics) , anatomy , chemistry , biology , pathology , medicine , programmed cell death , biochemistry , mathematics , paleontology , mathematical analysis
Purpose To determine the distribution of active caspase‐3 in rat eye lens epithelium. Methods In total, 120 sagittal sections from forty rats were assessed for active caspase‐3 labelling using immunohistochemistry. Lens epithelial cells were counted, and the fraction of active caspase‐3 labelled cells and their relative positions were identified in each section. Results Active caspase‐3 is present in normal lens epithelium. The active caspase‐3 expression was higher in the anterior pole of the lens. Probability of radial spatial distribution of labelling was fitted with a logistic model. The increase rate and the inflection point were estimated as CI (0.95) to 23 ± 3 cells and 114 ± 3 cells, respectively. Conclusion The gradually decreasing active caspase‐3 labelling from the anterior pole to the periphery suggests that active caspase‐3 may be involved in normal protein turnover caused by, for example, incident light.