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Collagen crosslinking of porcine sclera using genipin
Author(s) -
Liu TaiXiang,
Wang Zheng
Publication year - 2013
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.12172
Subject(s) - genipin , sclera , riboflavin , strain (injury) , chemistry , biomedical engineering , ophthalmology , medicine , anatomy , biochemistry , chitosan
. Purpose: To evaluate the effect of collagen cross‐linking induced by genipin in porcine sclera. Methods: Porcine cadaver eyes were treated with genipin at concentrations (by w/v) of 0.01%, 0.03%, 0.1%, 0.3%, 1.0% for 15 and 30 min. Riboflavin/ultraviolet A(UVA)‐treated and untreated samples were used as controls. After treatment, scleral strips of 4.0 × 10.0 mm were cut. Twenty‐four hours later, the stress‐strain parameters of the strips were measured using a biomaterial microtester. The stress and Young’s modulus at 8% strain were evaluated. Results: Compared with untreated groups, after treatment with genipin for 15 min, the stress was increased by 66–246%, depending on the concentration of genipin. As for the 30‐min groups, the stress was 171–444% higher than that of the control. The difference of the Young’s modulus between genipin 15‐min groups, except the 0.01% groups (p = 0.095), also had statistical significance (p < 0.05). The Young’s modulus had significant difference between the untreated group and the genipin 30‐min groups (all p < 0.05). Of 0.3% genipin for 15 min or 0.01% genipin for 30 min had a similar stress‐strain curve with those of eyes treated with the riboflavin/UVA group. The sclera exhibited a bluish colour which became deeper with increase concentration and cross‐linking time. Conclusions: Collagen cross‐linking induced by genipin could increase the biomechanical strength in porcine sclera. The effect depends on the concentration and treatment time of genipin.