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Presence and distribution of l‐kynurenine aminotransferases immunoreactivity in human cataractous lenses
Author(s) -
Rejdak Robert,
Oleszczuk Agnieszka,
Rummelt Carmen,
Turski Waldemar A.,
Choragiewicz Tomasz,
Nowomiejska Katarzyna,
Ksiazek Katarzyna,
Thaler Sebastian,
Zarnowski Tomasz,
Okuno Etsuo,
Grieb Pawel,
Zrenner Eberhart,
Kruse Friedrich,
Junemann Anselm G. M.
Publication year - 2013
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.12138
Subject(s) - kynurenine , distribution (mathematics) , ophthalmology , medicine , chemistry , biology , biochemistry , tryptophan , mathematics , amino acid , mathematical analysis
. Purpose: To investigate the presence and distribution of l ‐kynurenine aminotransferases immunoreactivity in human and animal lenses during cataract formation. Methods: Immunohistochemistry was conducted using polyclonal antibodies against KAT I, KAT II and KAT III on sections of 26 anterior capsules from patients undergoing surgical treatment of anterior subcapsular cataract (ASC) and 22 cataractous lenses from human eyes enucleated because of choroidal malignant melanoma. Additionally, the eyes of 11‐month‐old DBA/2J mice (6 eyes) were investigated (with KAT I and II). Ten clear human lenses and four BL6 mice lenses were used as controls. Spatial immunoreactivity patterns of enzymes were compared with Periodic Acid – Schiff (PAS)‐stained sections. Results: Immunohistochemical analysis revealed presence of KAT I, KAT II and KAT III in extracellular structures of all studied types of cataract in human eyes showing specific pattern of the stain. In cortical cataract, immunoreactivity was observed on cortical lens fibres. In nuclear cataract, KAT II revealed stronger and diffused staining than KAT I. Additionally, both KAT showed more pronounced staining at the edge of small clefts. In normal human lenses, KAT I, II and III, immunoreactivity was not observed. Presence of KAT I and KAT II in the intercellular substance of DBA/2J mice cataract was observed. In BL6 mice lenses without cataract, only weak KAT I and KAT II staining was observed. Conclusions: Presence of l ‐kynurenine aminotransferases in extracellular matrix (ECM) during human cataract formation suggests that products of l ‐kynurenine pathway might be involved in mechanisms of cataractogenesis.