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Expression of insulin‐like growth factor 1 receptor in rat retina following optic nerve injury
Author(s) -
Bu Shuyang,
Yu Gehua,
Xu Guoxu
Publication year - 2013
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.12096
Subject(s) - retina , optic nerve , insulin , medicine , receptor , insulin like growth factor , biology , ophthalmology , endocrinology , growth factor , microbiology and biotechnology , neuroscience
Abstract. Purpose: To investigate the apoptosis in retinal ganglion cells (RGCs) and insulin‐like growth factor 1 receptor (IGF‐1R) in the retina following optic nerve crush. Methods: Healthy Wistar rats ( N = 70) were divided into two groups: a normal control group and an optic nerve injury group. Immunohistochemistry and flow cytometry were performed to detect the expression of IGF‐1R and to measure the apoptosis of RGCs, respectively. Results: Immunohistochemistry revealed that at 1 hr after optic nerve injury, IGF‐1R immunoreactivity began to increase and reached a maximal level at 24 hr (p < 0.05), where it remained elevated up to 14 days after injury. RGC apoptosis in the normal control group was 0.53%, while the apoptosis rate in the optic nerve injury group increased over time. The apoptosis rate in the optic nerve injury group was 1.4% at 1 hr, 4.4% at 6 hr, 5.2% at 12 hr and reached a maximal level (8.5%) at 24 hr. Subsequently, the rate declined to 1.9% 7 days after injury and 0.9% 2 weeks after injury. Conclusion: The IGF‐1R immunereactivity in the retina increased after optic nerve injury. IGF‐1R may regulate the apoptosis and regeneration of RGCs at different stages after optic nerve injury.