Premium
Antioxidants reduce TGF‐beta2‐induced gene expressions in human optic nerve head astrocytes
Author(s) -
Yu Alice L.,
WelgeLussen Ulrich
Publication year - 2013
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.12013
Subject(s) - ctgf , fibronectin , vitamin , transforming growth factor , optic nerve , extracellular matrix , growth factor , transforming growth factor beta , connective tissue , chemistry , endocrinology , medicine , biology , pathology , microbiology and biotechnology , anatomy , receptor
. Purpose: The goal of the present study was to investigate whether the antioxidants vitamin E, vitamin C and vitamin B1 can reduce the transforming growth factor‐beta2 (TGF‐β2)‐induced gene expressions in cultured human optic nerve head (ONH) astrocytes. Methods: Cultured human ONH astrocytes were pretreated with different concentrations of vitamin E, vitamin C and vitamin B1 and then exposed to 1.0 ng/ml TGF‐β2 for 24 hr. Expression of the heat shock proteins Hsp27 and αB‐crystallin, the extracellular matrix (ECM) component fibronectin and the ECM‐modulating protein connective tissue growth factor (CTGF) was detected by immunohistochemistry or real‐time PCR analysis. Results: TGF‐β2 increased the expression of Hsp27, αB‐crystallin, fibronectin and CTGF in human ONH astrocytes. Pretreatment with different concentrations of vitamin E, vitamin C and vitamin B1 reduced the TGF‐β2‐stimulated gene expressions. Conclusion: In cultured human ONH astrocytes, the TGF‐β2‐stimulated gene expressions could be reduced by pretreatment with vitamin E, vitamin C and vitamin B1. Therefore, the use of antioxidants in glaucomatous optic neuropathy might be a promising approach to prevent TGF‐β2‐induced cellular changes in ONH astrocytes.