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Ocular surface microcirculation is better preserved with pulsatile versus continuous flow during cardiopulmonary bypass—An experimental pilot
Author(s) -
Kvernebo Anne Kari,
Miyamoto Takuma,
Drolsum Liv,
Moe Morten Carstens,
Måsøy SveinErik,
Sunagawa Gengo,
Dessoffy Raymond,
Karimov Jamshid H.,
Fukamachi Kiyotaka,
Kvernebo Knut
Publication year - 2022
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.14137
Subject(s) - pulsatile flow , microcirculation , cardiopulmonary bypass , blood flow , capillary action , medicine , oxygen saturation , cardiology , chemistry , oxygen , materials science , organic chemistry , composite material
Abstract Background Non‐pulsatile cardiopulmonary bypass (CPB) may induce microvascular dysregulation. In piglets, we compared ocular surface microcirculation during pulsatile versus continuous flow (CF) bypass. Methods Ocular surface microcirculation in small tissue volumes (~0.1 mm 3 ) at limbus (high metabolic rate) and bulbar conjunctiva (low metabolic rate) was examined in a porcine model using computer assisted video microscopy and diffuse reflectance spectroscopy, before and after 3 and 6 h of pulsatile ( n = 5 piglets) or CF ( n = 3 piglets) CPB. Functional capillary density, capillary flow velocity and microvascular oxygen saturation were quantified. Results At limbus, velocities improved with pulsatility ( p < 0.01) and deteriorated with CF ( p < 0.01). In bulbar conjunctiva, velocities were severely reduced with CF ( p < 0.01), accompanied by an increase in capillary density ( p < 0.01). Microvascular oxygen saturation decreased in both groups. Conclusion Ocular surface capillary densities and flow patterns are better preserved with pulsatile versus CF during 6 h of CPB in sleeping piglets.