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Using GRGDSPC peptides to improve re‐endothelialization of decellularized pancreatic scaffolds
Author(s) -
Wan Jian,
Wang Lei,
Huang Yan,
Fan Haowen,
Chen Chunqiu,
Yuan Xiaoqi,
Guo Yibing,
Yin Lu
Publication year - 2020
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.13602
Subject(s) - decellularization , umbilical vein , transplantation , chemistry , angiogenesis , tissue engineering , in vivo , biomedical engineering , scaffold , neovascularization , microbiology and biotechnology , in vitro , medicine , cancer research , biochemistry , surgery , biology
Engineering of functional vascularized pancreatic tissues offers an alternative way to solve the perpetual shortage of organs for transplantation. However, revascularization remains a major bottleneck in biological engineering, which limited the further clinical applications of this strategy. In this study, an efficient approach for enhancing re‐endothelialization of rat decellularized pancreatic scaffolds (DPS) was presented, by conjugating with GRGDSPC peptide to maximize coverage of the vessel walls with human umbilical vein endothelial cells (HUVECs). First, pancreas was perfused with 1% Triton X‐100 and 0.1% ammonium hydroxide to remove the cellular components. Subsequently, GRGDSPC was covalently coupled to the vasculature of DPS and re‐seeded with HUVECs via perfusion of the portal vein in the bioreactor. After the re‐endothelialized scaffolds were created, in vitro and in vivo experiments were undertaken to evaluate the angiogenesis. Our results demonstrated that GRGDSPC‐conjugated scaffolds could support the survival and accelerated the proliferation of HUVECs; angiogenesis was also significantly improved over untreated scaffolds. In conclusion, GRGDSPC‐conjugated scaffolds showed great potential for the generation of functional bioengineered pancreatic tissue suitable for long‐term transplantation.