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Teburu—Open source 3D printable bioreactor for tissue slices as dynamic three‐dimensional cell culture models
Author(s) -
Daneshgar Assal,
Tang Peter,
Remde Christopher,
Lommel Michael,
Moosburner Simon,
Kertzscher Ulrich,
Klein Oliver,
Weinhart Marie,
Pratschke Johann,
Sauer Igor M.,
Hillebrandt Karl H.
Publication year - 2019
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.13518
Subject(s) - bioreactor , biomedical engineering , open source , tissue engineering , materials science , chemistry , computer science , medicine , organic chemistry , software , programming language
Three‐dimensional tissue cultures are important models for the study of cell‐cell and cell‐matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system ( Teburu ), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three‐dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500‐µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu , as a semi‐open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions.