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In Vitro Dialysis of Cytokine‐Rich Plasma With High and Medium Cut‐Off Membranes Reduces Its Procalcific Activity
Author(s) -
Willy Kevin,
Hulko Michael,
Storr Markus,
Speidel Rose,
Gauss Julia,
Schindler Ralf,
Zickler Daniel
Publication year - 2017
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12884
Subject(s) - osteopontin , chemistry , dialysis tubing , in vitro , dialysis , calcification , cytokine , membrane , vascular smooth muscle , lipopolysaccharide , medicine , endocrinology , biochemistry , smooth muscle
Recently developed high‐flux (HF) dialysis membranes with extended permeability provide better clearance of middle‐sized molecules such as interleukins (ILs). Whether this modulation of inflammation influences the procalcific effects of septic plasma on vascular smooth muscle cells (VSMCs) is not known. To assess the effects of high cut‐off (HCO) and medium cut‐off (MCO) membranes on microinflammation and in vitro vascular calcification we developed a miniature dialysis model. Plasma samples from lipopolysaccharide‐spiked blood were dialyzed with HF, HCO, and MCO membranes in an in vitro miniature dialysis model. Afterwards, IL‐6 concentrations were determined in dialysate and plasma. Calcifying VSMCs were incubated with dialyzed plasma samples and vascular calcification was assessed. Osteopontin (OPN) and matrix Gla protein (MGP) were measured in VSMC supernatants. IL‐6 plasma concentrations were markedly lower with HCO and MCO dialysis. VSMC calcification was significantly lower after incubation with MCO‐ and HCO‐serum compared to HF plasma. MGP and OPN levels in supernatants were significantly lower in the MCO but not in the HCO group compared to HF. In vitro dialysis of cytokine‐enriched plasma samples with MCO and HCO membranes reduces IL‐6 levels. The induction of vascular calcification by cytokine‐enriched plasma is reduced after HCO and MCO dialysis.