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In Vivo Dual Fluorescence Imaging to Detect Joint Destruction
Author(s) -
Cho Hongsik,
Bhatti FazalUrRehman,
Lee Sangmin,
Brand David D.,
Yi AeKyung,
Hasty Karen A.
Publication year - 2016
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12685
Subject(s) - cartilage , matrix metalloproteinase , arthritis , rheumatoid arthritis , osteoarthritis , in vivo , type ii collagen , extracellular matrix , chemistry , matrix (chemical analysis) , alexa fluor , pathology , preclinical imaging , fluorescence , medicine , immunology , anatomy , biology , biochemistry , optics , physics , alternative medicine , microbiology and biotechnology , chromatography
Diagnosis of cartilage damage in early stages of arthritis is vital to impede the progression of disease. In this regard, considerable progress has been made in near‐infrared fluorescence (NIRF) optical imaging technique. Arthritis can develop due to various mechanisms but one of the main contributors is the production of matrix metalloproteinases (MMPs), enzymes that can degrade components of the extracellular matrix. Especially, MMP‐1 and MMP‐13 have main roles in rheumatoid arthritis and osteoarthritis because they enhance collagen degradation in the process of arthritis. We present here a novel NIRF imaging strategy that can be used to determine the activity of MMPs and cartilage damage simultaneously by detection of exposed type II collagen in cartilage tissue. In this study, retro‐orbital injection of mixed fluorescent dyes, MMPSense 750 FAST (MMP750) dye and Alexa Fluor 680 conjugated monoclonal mouse antibody immune‐reactive to type II collagen, was administered in the arthritic mice. Both dyes were detected with different intensity according to degree of joint destruction in the animal. Thus, our dual fluorescence imaging method can be used to detect cartilage damage as well as MMP activity simultaneously in early stage arthritis.