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Decellularization and Recellularization of Rat Livers With Hepatocytes and Endothelial Progenitor Cells
Author(s) -
Zhou Pengcheng,
Huang Yan,
Guo Yibing,
Wang Lei,
Ling Changchun,
Guo Qingsong,
Wang Yao,
Zhu Shajun,
Fan Xiangjun,
Zhu Mingyan,
Huang Hua,
Lu Yuhua,
Wang Zhiwei
Publication year - 2016
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12645
Subject(s) - decellularization , tissue engineering , scaffold , progenitor cell , extracellular matrix , biomedical engineering , adipose tissue , cellular infiltration , chemistry , pathology , microbiology and biotechnology , stem cell , anatomy , medicine , biology , inflammation , immunology , biochemistry
Whole‐organ decellularization has been identified as a promising choice for tissue engineering. The aim of the present study was to engineer intact whole rat liver scaffolds and repopulate them with hepatocytes and endothelial progenitor cells ( EPCs ) in a bioreactor. Decellularized liver scaffolds were obtained by perfusing T riton X ‐100 with ammonium hydroxide. The architecture and composition of the original extracellular matrix were preserved, as confirmed by morphologic, histological, and immunolabeling methods. To determine biocompatibility, the scaffold was embedded in the subcutaneous adipose layer of the back of a heterologous animal to observe the infiltration of inflammatory cells. Hepatocytes were reseeded using a parenchymal injection method and cultured by continuous perfusion. EPCs were reseeded using a portal vein infusion method. Morphologic and functional examination showed that the hepatocytes and EPCs grew well in the scaffold. The present study describes an effective method of decellularization and recellularization of rat livers, providing the foundation for liver engineering and the development of bioartificial livers.