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Self‐Assembled Infrapatellar Fat‐Pad Progenitor Cells on a Poly‐ε‐Caprolactone Film For Cartilage Regeneration
Author(s) -
Prabhakar Alisha,
Lynch Amy P.,
Ahearne Mark
Publication year - 2016
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12565
Subject(s) - chondrogenesis , cartilage , infrapatellar fat pad , extracellular matrix , hyaline cartilage , regeneration (biology) , glycosaminoglycan , chemistry , materials science , biomedical engineering , progenitor cell , matrix (chemical analysis) , osteoarthritis , anatomy , microbiology and biotechnology , stem cell , composite material , pathology , biochemistry , medicine , biology , articular cartilage , alternative medicine
Cartilage defects resulting from osteoarthritis ( OA ) or physical injury can severely reduce the quality of life for sufferers. Current treatment options are costly and not always effective in producing stable hyaline cartilage. Here we investigated a new treatment option that could potentially repair and regenerate damaged cartilage tissue. This novel approach involves the application of infrapatellar fat‐pad derived chondroprogenitor cells onto a mechanically stable biodegradable polymer film that can be easily implanted into a defect site. Poly‐ε‐caprolactone ( PCL ) films were fabricated via solvent casting in either acetone or chloroform. The hydrophobicity, mechanical properties, and surface morphology of the films were examined. Progenitor cells from infrapatellar fat‐pad were isolated, expanded, and then seeded onto the films. The cells were allowed to self‐assemble on films, and these were then cultured in a chemically defined chondrogenic media for 28 days. The self‐assembled tissue was characterized via histological staining, gene expression analysis, immunohistochemistry, and biochemical analysis. Chondrogenic differentiation was induced to generate a cartilaginous matrix upon the films. Despite differences between in the appearance, surface morphology, and mechanical properties of the films cast in chloroform or acetone, both methods produced tissues rich in sulfated glycosaminoglycan and collagen, although the extracellular matrix produced on chloroform‐cast films appeared to contain more collagen type II and less collagen type I than acetone‐cast films. These self‐assembled constructs have the potential to be implanted into defect sites as a potential treatment for cartilage defect regeneration.