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Fluid–Structure Interactions Analysis of Shear‐Induced Modulation of a Mesenchymal Stem Cell: An Image‐Based Study
Author(s) -
Vaez Ghaemi Roza,
Vahidi Bahman,
Sabour Mohammad Hossein,
Haghighipour Nooshin,
Alihemmati Zakieh
Publication year - 2016
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12547
Subject(s) - mesenchymal stem cell , multiphysics , shear stress , fluid dynamics , cell , biological system , computational model , flow (mathematics) , microchannel , finite element method , materials science , biomedical engineering , stem cell , microbiology and biotechnology , biophysics , mechanics , chemistry , nanotechnology , computer science , structural engineering , simulation , biology , physics , engineering , biochemistry
Although effects of biochemical modulation of stem cells have been widely investigated, only recent advances have been made in the identification of mechanical conditioning on cell signaling pathways. Experimental investigations quantifying the micromechanical environment of mesenchymal stem cells ( MSCs ) are challenging while computational approaches can predict their behavior due to in vitro stimulations. This study introduces a 3 D cell‐specific finite element model simulating large deformations of MSCs . Here emphasizing cell mechanical modulation which represents the most challenging multiphysics phenomena in sub‐cellular level, we focused on an approach attempting to elicit unique responses of a cell under fluid flow. Fluorescent staining of MSCs was performed in order to visualize the MSC morphology and develop a geometrically accurate model of it based on a confocal 3 D image. We developed a 3 D model of a cell fixed in a microchannel under fluid flow and then solved the numerical model by fluid–structure interactions method. By imposing flow characteristics representative of vigorous in vitro conditions, the model predicts that the employed external flow induces significant localized effective stress in the nucleo‐cytoplasmic interface and average cell deformation of about 40%. Moreover, it can be concluded that a lower strain level is made in the cell by the oscillatory flow as compared with steady flow, while same ranges of effective stress are recorded inside the cell in both conditions. The deeper understanding provided by this study is beneficial for better design of single cell in vitro studies.