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Heparin‐Conjugated Poly(Lactic‐Co‐Glycolic Acid) Nanospheres Enhance Large‐Wound Healing by Delivering Growth Factors in Platelet‐Rich Plasma
Author(s) -
La WanGeun,
Yang Hee Seok
Publication year - 2015
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12389
Subject(s) - wound healing , platelet rich plasma , glycolic acid , growth factor , chemistry , fibrin , heparin , angiogenesis , fibroblast growth factor , regeneration (biology) , platelet , pharmacology , fibroblast , platelet derived growth factor receptor , platelet derived growth factor , lactic acid , surgery , biochemistry , immunology , medicine , cancer research , microbiology and biotechnology , in vitro , biology , bacteria , receptor , genetics
Platelet‐rich plasma ( PRP ) contains many growth factors that are involved in tissue regeneration processes. For successful tissue regeneration, protein growth factors require a delivery vehicle for long‐term and sustained release to a defect site in order to maintain their bioactivity. Previously, we showed that heparin‐conjugated poly(lactic‐co‐glycolic acid) nanospheres ( HCPNs ) can provide long‐term delivery of growth factors with affinity for heparin. In this study, we hypothesize that treatment of a skin wound with a mixture of PRP and HCPNs would provide long‐term delivery of several growth factors contained in PRP to promote the skin wound healing process with preservation of bioactivity. The release of platelet‐derived growth factor‐ BB ( PDGF ‐ BB ), contained in PRP , from HCPN with fibrin gel ( FG ) showed a prolonged release period versus a PRP mixture with FG alone ( FG ‐ PRP ). Also, growth factors released from PRP with HCPN and FG showed sustained human dermal fibroblast growth for 12 days. Full‐thickness skin wound treatment in mice with FG ‐ HCPN ‐ PRP resulted in much faster wound closure as well as dermal and epidermal regeneration at day 9 compared with treatment with FG ‐ HCPN or FG ‐ PRP . The enhanced wound healing using FG ‐ HCPN ‐ PRP may be due to the prolonged release not only of PDGF ‐ BB but also of other growth factors in the PRP . The delivered growth factors accelerated angiogenesis at the wound site.