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Effects of Liposome‐Encapsulated Hemoglobin on Learning Ability in T okai High‐Avoider Rat After Total Brain Ischemia and Reperfusion
Author(s) -
Kawaguchi Akira T.,
Endo Hitoshi,
Aikawa Hiroyuki,
Yamano Mariko,
Kawaguchi Yo,
Haida Munetaka,
Watanabe Tetsu
Publication year - 2014
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12352
Subject(s) - morris water navigation task , ischemia , saline , hippocampus , medicine , hemoglobin , anesthesia , amygdala
Liposome‐encapsulated hemoglobin with low O 2 ‐affinity ( l ‐ LEH ) was shown to be protective in focal brain ischemia and reperfusion ( I / R ) in rats and primates. We tested l ‐ LEH in the transient whole brain ischemia in the T okai high‐avoider rat ( THA ), which has been selected, mated, and bred over 77 generations for a high and consistent learning ability determined by the S idman avoidance test ( SAT ). Young/naïve (before SAT ) and adult/parent (after SAT ) THA rats underwent acute and complete four‐vessel occlusion in the chest for 3 or 5 min, administration of 2 mL/kg of l ‐ LEH , saline, or homologous washed red blood cells ( RBC s), reperfusion, and resuscitation. One week later, all rats underwent SAT , open‐field behavioral observation, M orris water maze tests, and morphological study. Whereas young/naïve rats treated with l ‐ LEH retained a rapid and consistent learning curve as in nonischemic controls, THA rats treated with RBCs or saline had retarded learning response on SAT as well as reduced cellularity in the amygdala. Adult/parent rats with established memory on SAT maintained perfect achievement even after I / R . In contrast, l ‐ LEH ‐treated rats showed no better performance on M orris water maze (function) or cellularity of the CA 1 sector of the hippocampus (morphology) compared with the rats treated with RBCs . Although task performance on SAT and M orris water maze appeared antithetical, morphological observations corresponded to the respective functions, suggesting that l ‐ LEH was protective only for the amygdala on SAT tasks but not for the CA 1 sector of the hippocampus on spatial orientation as in our previous studies on focal brain I / R , where the cortex was preserved better than basal ganglia.