Premium
Real‐Time Trafficking of PEG ylated Liposomes in the Rodent Focal Brain Ischemia Analyzed by Positron Emission Tomography
Author(s) -
Fukuta Tatsuya,
Ishii Takayuki,
Asai Tomohiro,
Nakamura Genki,
Takeuchi Yoshihito,
Sato Akihiko,
Agato Yurika,
Shimizu Kosuke,
Akai Shuji,
Fukumoto Dai,
Harada Norihiro,
Tsukada Hideo,
Kawaguchi Akira T.,
Oku Naoto
Publication year - 2014
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12350
Subject(s) - liposome , medicine , ischemia , positron emission tomography , drug delivery , cerebral blood flow , peg ratio , brain ischemia , perfusion , occlusion , pharmacology , middle cerebral artery , anesthesia , nuclear medicine , chemistry , biochemistry , organic chemistry , finance , economics
A liposomal drug delivery system was previously applied to ischemic brain model rats for the treatment of brain ischemia, and we observed that 100‐nm‐sized liposomes could extravasate and accumulate in the ischemic brain region even when cerebral blood flow was markedly reduced in permanent middle cerebral artery occlusion ( p ‐ MCAO ) model rats. In the present study, we investigated the real‐time cerebral distribution of polyethylene glycol ( PEG )‐modified liposomes ( PEG ‐liposomes) labeled with 1‐[ 18 F ]fluoro‐3,6‐dioxatetracosane in p ‐ MCAO rats by positron emission tomography ( PET ). [ 18 F ]‐Labeled PEG‐liposomes were intravenously injected into p ‐ MCAO rats 1 h after the onset of occlusion, and then a PET scan was performed for 2 h. The PET scan showed that the signal intensity of [ 18 F ] gradually increased in the ischemic region despite the drastic reduction in cerebral perfusion, suggesting that PEG ‐liposomes had accumulated in and around the ischemic region. Therefore, drug delivery to the ischemic region by use of liposomes would be possible under ischemic conditions, and a liposomal drug delivery system could be a promising strategy for protecting the ischemic brain from damage before recovery from ischemia.