Premium
Activation of Basophils Is a New and Sensitive Marker of Biocompatibility in Hemodialysis
Author(s) -
Aljadi Zenib,
Mansouri Ladan,
Nopp Anna,
Paulsson Josefin M.,
Winqvist Ola,
Russom Aman,
Ståhl Mårten,
Hylander Britta,
Jacobson Stefan H.,
Lundahl Joachim
Publication year - 2014
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12297
Subject(s) - cd63 , hemodialysis , chemistry , integrin alpha m , immunology , basophil activation , basophil , innate immune system , epitope , antibody , medicine , receptor , biochemistry , immunoglobulin e , microrna , microvesicles , gene
The hemodialysis procedure involves contact between peripheral blood and the surface of dialyzer membranes, which may lead to alterations in the pathways of innate and adaptive immunity. We aimed to study the effect of blood–membrane interaction on human peripheral basophils and neutrophils in hemodialysis with high‐ and low‐permeability polysulfone dialyzers. The surface expression of CD 203c (basophil selection marker) and CD 63 (activation marker) after activation by the bacterial peptide formyl‐methionyl‐leucyl‐phenylalanine ( fMLP ) or anti‐ F cε receptor I ( F cε RI ) antibody and the absolute number of basophils was investigated before and after hemodialysis with each of the dialyzers. Moreover, the expression on neutrophils of CD 11b, the CD 11b active epitope, and CD 88 was analyzed in the same groups of individuals. The expression of CD 63 in basophils following activation by fMLP was significantly higher in the patient group compared with that in healthy controls, but no differences were observed after activation by anti‐ F cε RI . During the hemodialysis procedure, the low‐flux membrane induced up‐regulation of CD 63 expression on basophils, while passage through the high‐flux membrane did not significantly alter the responsiveness. In addition, the absolute number of basophils was unchanged after hemodialysis with either of the dialyzers and compared with healthy controls. We found no significant differences in the expression of the neutrophil activation markers ( CD 11b, the active epitope of CD 11b, and CD 88) comparing the two different dialyzers before and after dialysis and healthy controls. Together, these findings suggest that alterations in basophil activity may be a useful marker of membrane bioincompatibility in hemodialysis.