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Promotion of Full‐Thickness Wound Healing Using Epigallocatechin‐3‐ O ‐Gallate/Poly (Lactic‐ C o‐ G lycolic Acid) Membrane as Temporary Wound Dressing
Author(s) -
Kim HyeLee,
Lee JeongHyun,
Kwon Byeong Ju,
Lee Mi Hee,
Han DongWook,
Hyon SuongHyu,
Park JongChul
Publication year - 2014
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12190
Subject(s) - plga , glycolic acid , wound healing , membrane , chemistry , epigallocatechin gallate , lactic acid , gallate , angiogenesis , cytotoxicity , pharmacology , polyphenol , biochemistry , cancer research , surgery , nuclear chemistry , in vitro , medicine , bacteria , antioxidant , biology , genetics
Abstract Epigallocatechin‐3‐ O ‐gallate ( EGCG ) is a major polyphenolic compound in green tea. It has been known that EGCG regulates the secretion of cytokines and the activation of skin cells during wound healing. In this study, various concentrations of EGCG were added to the electrospun membranes composed of poly (lactic‐co‐glycolic acid) ( PLGA ), and its healing effects on full‐thickness wounds created in nude mice were investigated. The electrospun membranes containing 5 wt% EGCG (5 EGCG / PLGA membrane) exhibited cytotoxicity in human dermal fibroblasts ( HDFs ) as HDF morphologies were transformed on them. In the animal study, cell infiltration of mice treated with electrospun membranes containing 1 wt% EGCG (1 EGCG / PLGA membrane) significantly increased after 2 weeks. The immunoreactivity of K i‐67 (re‐epithelialization at the wound site) and CD 31 (formation of blood vessels) also increased in the mice treated with 1 EGCG / PLGA membranes in comparison with the mice treated with PLGA membranes. These results suggest that 1 EGCG / PLGA can enhance wound healing in full thickness by accelerating cell infiltration, re‐epithelialization, and angiogenesis.