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Primary and Secondary Immune Responses to Keyhole Limpet Hemocyanin in Rats After Infusion of Hemoglobin Vesicle, an Artificial Oxygen Carrier
Author(s) -
Fujihara Mitsuhiro,
Takahashi Daisuke,
Abe Hideki,
Sakai Hiromi,
Horinouchi Hirohisa,
Kobayashi Koichi,
Ikeda Hisami,
Azuma Hiroshi
Publication year - 2014
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12148
Subject(s) - keyhole limpet hemocyanin , immune system , immunology , antibody , hemoglobin , in vivo , hemocyanin , spleen , biology , chemistry , biochemistry , microbiology and biotechnology
Hemoglobin vesicles ( HbVs ), artificial oxygen carriers encapsulating concentrated Hb solution on phospholipid vesicles (liposomes), are promising candidates for clinically useful transfusion. Although HbV infusion transiently suppressed the proliferative response of rat splenic T ‐cells to concanavalin A or keyhole limpet hemocyanin ( KLH ), a T ‐cell‐dependent antigen, in ex vivo culture conditions, HbV infusion did not affect the primary IgG antibody response. We extended our assessment of the effects of HbV infusion on the systemic immune response using primary and secondary responses to KLH in rats. We observed that the generation of primary anti‐ KLH IgM antibody in HbV ‐infused rats was not suppressed but was instead higher than those in saline‐infused rats. Furthermore, HbV infusion did not suppress the increase of IgG subclass of KLH antibody in secondary response. The T cell response to KLH of bulk spleen cells, as derived from 2–3 months after secondary KLH immunization, was unaffected by infusion of HbV , suggesting that HbV loading has no suppressive effect on homeostatic survival of memory T ‐cells against KLH . These results indicate that HbV is highly biocompatible in systemic immune responses in rats.