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Continuous Monitoring of Inflammation Biomarkers During Simulated Cardiopulmonary Bypass Using a Microfluidic Immunoassay Device—A Pilot Study
Author(s) -
Sasso Lawrence A.,
Aran Kiana,
Guan Yulong,
Ündar Akif,
Zahn Jeffrey D.
Publication year - 2013
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.12021
Subject(s) - immunoassay , cardiopulmonary bypass , medicine , microfluidics , inflammation , immunology , materials science , antibody , nanotechnology
This work demonstrates the use of a continuous online monitoring system for tracking systemic inflammation biomarkers during cardiopulmonary bypass ( CPB ) procedures. The ability to monitor inflammation biomarkers during CPB will allow surgical teams to actively treat inflammation and reduce harmful effects on postoperative morbidity and mortality, enabling improved patient outcomes. A microfluidic device has been designed which allows automation of the individual processing steps of a microbead immunoassay to allow continuous tracking of antigen concentrations. Preliminary experiments have demonstrated that the results produced by the microimmunoassay are comparable to results produced from a standard enzyme‐linked immunosorbent assay ( r = 0.98). Additionally, integration of the assay with a simulated CPB circuit has been demonstrated with temporal tracking of C 3a concentrations within blood continuously sampled from the circuit. The presented work describes the motivation, design challenges, and preliminary experimental results of this project.