Open AccessTranslational research aiming to improve survival of ovarian tissue transplants using adipose tissue‐derived stem cells
Author(s) -
Dolmans MarieMadeleine,
Cacciottola Luciana,
Amorim Christiani A.,
Manavella Diego
Publication year - 2019
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1111/aogs.13610
Subject(s) - transplantation , medicine , adipose tissue , stem cell , cd90 , andrology , cd31 , ovarian tissue cryopreservation , angiogenesis , cd34 , fertility preservation , surgery , biology , microbiology and biotechnology , fertility , population , environmental health
There is now sufficient evidence to support the feasibility and efficacy of ovarian tissue (OT) cryopreservation and transplantation for both fertility preservation and restoration purposes. However, there are still issues to address regarding the grafting procedure itself, since transplanted tissue suffers massive follicle loss in the early post‐grafting period. To improve follicle survival after transplantation, our group recently developed a two‐step transplantation technique for OT transplantation in a xenografting model using adipose tissue‐derived stem cells ( ASC s). The aim of this narrative review is to describe and discuss the previously reported findings. ASC s were initially characterized by flow cytometry as positive for CD 29, CD 44, CD 73, CD 90, CD 105 and CD 166 (>95%) and negative for CD 34, CD 14, CD 31, CD 45 and Lin1. ASC s were used in a model of xenotransplantation, were they were embedded in a fibrin scaffold and transplanted to the peritoneum of immunodeficient mice. The goal of the first step was to increase levels of partial pressure of oxygen ( pO 2 ) and revascularization in the peritoneal transplantation site for further OT transplantation. As ASC s showed the ability to differentiate into endothelial‐like cells and vessels in our model, OT transplantation was then performed with ASC grafts in a controlled experiment. At 7 days post‐transplantation, the ASC group showed: (1) significantly higher pO 2 levels; (2) significantly greater human and murine CD 34‐positive endothelial areas; (3) significantly higher primordial follicle survival rates; (4) and significantly lower numbers of apoptotic follicles compared with the control group. Our research model demonstrates that by adding ASC s to a fibrin scaffold before OT transplantation, faster and better graft reoxygenation and revascularization may be obtained, resulting in increased follicle survival and reduced follicle apoptosis.