Open AccessToll‐like receptor 2 gene polymorphisms in Korean women with human papillomavirus‐related cervical neoplasia
Author(s) -
Kim Jin Hwi,
Key Eun Young,
Song Min Jong,
Lee Sung Jong,
Kim Chan Joo,
Hur Soo Young,
Park Tae Chul,
Kim Tae Gyu,
Park Jong Sup
Publication year - 2016
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1111/aogs.12895
Subject(s) - genotype , odds ratio , medicine , tlr2 , hpv infection , allele , restriction fragment length polymorphism , gastroenterology , polymerase chain reaction , oncology , immunology , gynecology , cervical cancer , gene , genetics , receptor , biology , tlr4 , cancer
The aim of this study was to investigate the association between Toll‐like receptor 2 ( TLR 2 ) gene polymorphisms and human papillomavirus ( HPV )‐related cervical neoplasia in Korean women. Material and methods Peripheral blood samples collected from 127 patients with HPV ‐related cervical neoplasia and 175 healthy women were genotyped for the TLR 2 −16934, +1350, intron1, and 3′ untranslated region ( UTR ) polymorphisms using the polymerase chain reaction and restriction fragment length polymorphism method. Results The TLR 2 −16934 A/A, intron1 A/A, and +1350 T/C genotypes were more frequent in patients than in controls [odds ratio ( OR ) = 2.1, 95% CI = 1.302–3.475, p = 0.002; OR = 1.9, 95% CI = 1.168–3.169, p = 0.010; and OR = 1.9, 95% CI = 1.211–3.123, p = 0.006, respectively]. The frequencies of the TLR 2 + 1350 C and 3′ UTR G alleles were also higher in patients ( OR = 2.0, 95% CI = 1.236–3.121, p = 0.004 and OR = 1.7, 95% CI = 1.005–3.076, p = 0.046, respectively). The genotype frequencies of TLR 2 −16934 A/A and intron1 A/A increased with increasing oncogenic risk of the HPV genotype, as follows. low‐risk type < high‐risk type < HPV ‐16 and/or HPV ‐18 type ( p = 0.008). Conclusions Our study provides the first evidence that TLR 2 gene polymorphisms are associated with high‐risk type HPV ‐related cervical neoplasia and may play an important role in susceptibility to HPV infection. Further large‐scale and functional studies are needed to confirm the role of TLR 2 gene polymorphisms in HPV ‐related cervical neoplasia.