Open AccessRole of SOCS 3 in the Jak/stat3 pathway in the human placenta: different mechanisms for preterm and term labor
Author(s) -
Zhou Xin,
Jiang Ziyan,
Zou Yanfen,
Yin Yin,
Zuo Qing,
Sun Lizhou
Publication year - 2015
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1111/aogs.12708
Subject(s) - trophoblast , socs3 , andrology , placenta , tunel assay , apoptosis , stat3 , medicine , downregulation and upregulation , western blot , biology , fetus , pregnancy , gene , biochemistry , genetics
To identify changes in interleukin ( IL )‐6 levels and its pathway (Jak/stat3) in the human placenta during preterm and term labor, placental tissues were collected from primiparous women who underwent vaginal deliveries or cesarean sections in our hospital. The women were divided into three groups: preterm labor ( n = 15), term labor ( n = 15), and term not in labor ( n = 15). Material and methods IL ‐6 levels were detected by ELISA in placental supernatant, and p‐ STAT 3 and SOCS 3 protein was detected by Western blot. TUNEL was used to detect apoptosis in trophoblasts. HTR ‐8/ SV neo cells were cultured after stimulation with IL ‐6, and we measured p‐ STAT 3, SOCS 3, and the rate of apoptosis. Results Expression of p‐ STAT 3 and SOCS 3 in the placenta and trophoblast cells showed that IL ‐6 levels were highest in the preterm labor group and lowest in the term not in labor group. The highest expression of placental SOCS 3 protein was observed in the preterm labor group. More apoptotic cells were found in the preterm labor group than in the other two groups by TUNEL . SOCS 3 and p‐ STAT 3 expression was significantly upregulated after stimulation by IL ‐6 in trophoblast cells in a dose‐dependent manner. However, p‐ STAT 3 was significantly decreased after 50 ng/ mL and 100 ng/ mL IL ‐6 for 72 h. A significant increase of apoptosis was observed with treatment of 50 ng/ mL IL ‐6 in HTR ‐8/ SV neo cells. Conclusions The role of the SOCS 3 protein in the Jak/stat3 pathway is to mediate different mechanisms for preterm and term labor processes in the placenta.